EuroClonality Publications
PCR GeneScan Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes for Clonality Diagnostics in Suspect Lymphoproliferations
Boone E, Groenen PJTA, Langerak AW.
Reference: Methods Mol Biol. 2025; 2865: 77-102.
Link: https://pubmed.ncbi.nlm.nih.gov/39424721/
EuroClonality-NGS Recommendations for Evaluation of B-Cell Clonality Analysis by Next-Generation Sequencing: A Structured Approach with the DEPART Algorithm
van den Brand M, Möbs M, Otto F, Kroeze LI, Gonzalez de Castro D, Stamatopoulos K, Davi F, Bravetti C, Kolijn PM, Vlachonikola E, Stewart JP, Pott C, Hummel M, Darzentas N, Langerak AW, Fend F, Groenen PJTA.
Reference: J Mol Diagn. 2023;25:729-739.
Link: https://pubmed.ncbi.nlm.nih.gov/37467928/
Clonotype definitions for immunogenetic studies: proposals from the EuroClonality NGS Working Group
Sofou E, Vlachonikola E, Zaragoza-Infante L, Brüggemann M, Darzentas N, Groenen PJTA, Hummel M, Macintyre EA, Psomopoulos F, Davi F, Langerak AW, Stamatopoulos K.
Reference: Leukemia. 2023 Aug 37;1750-1752.
Link: https://pubmed.ncbi.nlm.nih.gov/37391484/
Extranodal marginal zone lymphoma clonotypes are detectable prior to eMZL diagnosis in tissue biopsies and peripheral blood of Sjögren's syndrome patients through immunogenetics
Kolijn PM, Huijser E, Wahadat MJ, van Helden-Meeuwsen CG, van Daele PLA, Brkic Z, Rijntjes J, Hebeda KM, Groenen PJTA, Versnel MA, Thurlings RM, Langerak AW
Reference: Front Oncol. 2023;13:1130686.
Link: https://pubmed.ncbi.nlm.nih.gov/37035202/
Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology
Groenen PJTA, van den Brand M, Kroeze LI, Amir AL, Hebeda KM.
Reference: Front Oncol. 2023 Feb 8;13:1107171.
Link: https://pubmed.ncbi.nlm.nih.gov/36845702/
A novel next-generation sequencing capture-based strategy to report somatic hypermutation status using genomic regions downstream to immunoglobulin rearrangements
McCafferty N, Stewart JP, Darzentas N, Gazdova J, Catherwood M, Stamatopoulos K, Langerak AW, Gonzalez D
Reference: Haematologica . 2023;108:1313-1321.
Link: https://pubmed.ncbi.nlm.nih.gov/36579446/
The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease
Langerak AW
Reference: Methods Mol Biol. 2022;2453:1-5.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_1
Next-generation sequencing-based clonality detection of immunoglobulin gene rearrangements in B-cell lymphoma
van Bladel DAG, van der Last-Kempkes JLM, Scheijen B, Groenen PJTA; EuroClonality Consortium.
Reference: Methods Mol Biol. 2022;2453:7-42.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_2
One-Step Next-Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia
Villarese P, Abdo C, Bertrand M, Thonier F, Giraud M, Salson M, Macintyre E
Reference: Methods Mol Biol. 2022;2453:43-59
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_3
Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq
van der Velden VHJ, Bastian L, Brüggemann M, Hartmann AM, Darzentas N.
Reference: Methods Mol Biol. 2022;2453:61-77
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_4
Quality control for IG /TR marker identification and MRD analysis
Fronkova E, Svaton M, Trka J
Reference: Methods Mol Biol. 2022;2453:91-99.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_6
cfDNA-Based NGS IG Analysis in Lymphoma
Pott C, Kotrova M, Darzentas N, Brüggemann M, Khouja M; EuroClonality-NGS Working Group
Reference: Methods Mol Biol. 2022;2453:101-117.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_7
Targeted locus amplification as marker screening approach to detect immunoglobulin (IG) translocations in B-cell non-hodgkin lymphomas
Genuardi E, Alessandria B, Civita AM, Ferrero S.
Reference: Methods Mol Biol. 2022;2453:119-132.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_8
Immunoglobulin/T cell receptor capture strategy for comprehensive immunogenetics
Stewart JP, Gazdova J, Srivastava S, Revolta J, Harewood L, Maurya M, Darzentas N, Gonzalez D
Reference: Methods Mol Biol. 2022;2453:133-152.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_9
Immunoglobulin gene mutational status assessment by next generation sequencing in chronic lymphocytic Leukemia
Langlois de Septenville A, Boudjoghra M, Bravetti C, Armand M, Salson M, Giraud M, Davi F
Reference: Methods Mol Biol. 2022;2453:153-167.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_10
ARResT/Interrogate Immunoprofiling platform: concepts, workflows, and insights
Darzentas N
Reference: Methods Mol Biol. 2022;2453:571-584.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_26
Purpose-built immunoinformatics for BcR IG/TR repertoire data analysis
Galigalidou C, Zaragoza-Infante L, Chatzidimitriou A, Stamatopoulos K, Psomopoulos F, Agathangelidis A
Reference: Methods Mol Biol. 2022;2453:585-603.
Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_27
Next-generation sequencing technology to identify minimal residual disease in lymphoid malignancies
Kotrova M, Darzentas N, Pott C, Brüggemann M; EuroClonality-NGS Working Group
Reference: Methods Mol Biol. 2021;2185:95-111.
Link: https://pubmed.ncbi.nlm.nih.gov/33165845/
Next-generation sequencing-based clonality assessment of Ig Gene Rearrangements: A multicenter validation study by EuroClonality-NGS
van den Brand M, Rijntjes J, Möbs M, Steinhilber J, van der Klift MY, Heezen KC, Kroeze LI, Reigl T, Porc J, Darzentas N, Luijks JACW, Scheijen B, Davi F, ElDaly H, Liu H, Anagnostopoulos I, Hummel M, Fend F, Langerak AW, Groenen PJTA; EuroClonality-NGS Working Group.
Reference: J Mol Diagn. 2021;23:1105-1115.
Link: https://pubmed.ncbi.nlm.nih.gov/34186174/
Potential and pitfalls of whole transcriptome-based immunogenetic marker identification in acute lymphoblastic leukemia; a EuroMRD and EuroClonality-NGS Working Group study.
van der Velden VHJ, Brüggemann M, Cazzaniga G, Scheijen B, Tops B, Trka J, Pal K, Hänzelmann S, Fazio G, Songia S, Langerak AW, Darzentas N; EuroMRD; EuroClonality-NGS Working Group.
Reference: Leukemia. 2021;35:924-928.
Link: https://pubmed.ncbi.nlm.nih.gov/33608635/
Validation of the EuroClonality-NGS DNA capture panel as an integrated genomic tool for lymphoproliferative disorders
Stewart JP, Gazdova J, Darzentas N, Wren D, Proszek P, Fazio G, Songia S, Alcoceba M, Sarasquete ME, Villarese P, van der Klift MY, Heezen KC, McCafferty N, Pal K, Catherwood M, Kim CS, Srivastava S, Kroeze LI, Hodges E, Stamatopoulos K, Klapper W, Genuardi E, Ferrero S, van den Brand M, Cazzaniga G, Davi F, Sutton LA, Garcia-Sanz R, Groenen PJTA, Macintyre E, Brüggemann M, Pott C, Langerak AW, Gonzalez D; EuroClonality-NGS Working Group.
Reference: Blood Adv. 2021;5:3188-3198.
Link: https://pubmed.ncbi.nlm.nih.gov/34424321/
Evaluation of a worldwide EQA scheme for complex clonality analysis of clinical lymphoproliferative cases demonstrates a learning effect
Keppens C, Boone E, Gameiro P, Tack V, Moreau E, Hodges E, Evans P, Brüggemann M, Carter I, Lenze D, Sarasquete ME, Möbs M, Liu H, Dequeker EMC, Groenen PJTA
Reference: Virchows Arch. 2021;479:365-376.
Link: https://pubmed.ncbi.nlm.nih.gov/33686511/
Immunoglobulin gene analysis in chronic lymphocytic leukemia in the era of next generation sequencing
Davi F, Langerak AW, de Septenville AL, Kolijn PM, Hengeveld PJ, Chatzidimitriou A, Bonfiglio S, Sutton LA, Rosenquist R, Ghia P, Stamatopoulos K; ERIC, the European Research Initiative on CLL, and the EuroClonality-NGS Working Group
Reference: Leukemia. 2020;34:2545-2551.
Link: https://pubmed.ncbi.nlm.nih.gov/32561841/
Automation of amplicon-based library preparation for next-generation sequencing by centrifugal microfluidics
Hess JF, Kotrová M, Calabrese S, Darzentas N, Hutzenlaub T, Zengerle R, Brüggemann M, Paust N
Reference: Anal Chem. 2020;92:12833-12841
Memento for interprofessional learning
Groenen PJTA, Langerak AW, Fend F, van Krieken JHJM
Reference: Virchows Arch. 2020;477:755-756.
Link: https://pubmed.ncbi.nlm.nih.gov/32270299/
PCR GeneScan and heteroduplex analysis of rearranged immunoglobulin or T-cell receptor genes for clonality diagnostics in suspect lymphoproliferations
Boone E, Heezen KC, Groenen PJTA, Langerak AW; EuroClonality Consortium
Reference: Methods Mol Biol. 2019;1956:77-103.
Link: https://pubmed.ncbi.nlm.nih.gov/30779031/
Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study
Brüggemann M, Kotrová M, Knecht H, Bartram J, Boudjogrha M, Bystry V, Fazio G, Froňková E, Giraud M, Grioni A, Hancock J, Herrmann D, Jiménez C, Krejci A, Moppett J, Reigl T, Salson M, Scheijen B, Schwarz M, Songia S, Svaton M, van Dongen JJM, Villarese P, Wakeman S, Wright G, Cazzaniga G, Davi F, García-Sanz R, Gonzalez D, Groenen PJTA, Hummel M, Macintyre E, Stamatopoulos K, Pott C, Trka J, Darzentas N, Langerak AW; EuroClonality-NGS working group
Reference: Leukemia. 2019;33:2241-2253.
Link: https://www.ncbi.nlm.nih.gov/pubmed/31243313
A new and simple TRG multiplex PCR assay for assessment of T-cell clonality: A comparative study from the EuroClonality Consortium
Armand M, Derrieux C, Beldjord K, Wabeke T, Lenze D, Boone E, Brüggemann M, Evans PAS, Gameiro P, Hummel M, Villarese P, Groenen PJTA, Langerak AW, Macintyre E, Davi F
Reference: Hemasphere. 2019;3:e255.
Link: https://pubmed.ncbi.nlm.nih.gov/31723840/
Quality control and quantification in IG/TR next-generation sequencing marker identification: protocols and bioinformatic functionalities by EuroClonality-NGS
Knecht H, Reigl T, Kotrová M, Appelt F, Stewart P, Bystry V, Krejci A, Grioni A, Pal K, Stranska K, Plevova K, Rijntjes J, Songia S, Svatoň M, Froňková E, Bartram J, Scheijen B, Herrmann D, García-Sanz R, Hancock J, Moppett J, van Dongen JJM, Cazzaniga G, Davi F, Groenen PJTA, Hummel M, Macintyre E, Stamatopoulos K, Trka J, Langerak AW, Gonzalez D, Pott C, Brüggemann M, Darzentas N; EuroClonality-NGS Working Group
Reference: Leukemia. 2019;33:2254-2265 (2019 Jun 21)
Link: https://www.ncbi.nlm.nih.gov/pubmed/31227779
Next-generation sequencing of immunoglobulin gene rearrangements for clonality assessment: a technical feasibility study by EuroClonality-NGS
Scheijen B, Meijers RWJ, Rijntjes J, van der Klift MY, Möbs M, Steinhilber J, Reigl T, van den Brand M, Kotrová M, Ritter JM, Catherwood MA, Stamatopoulos K, Brüggemann M, Davi F, Darzentas N, Pott C, Fend F, Hummel M, Langerak AW, Groenen PJTA; EuroClonality-NGS Working Group
Reference: Leukemia 2019;33:2227-2240.
Link: https://www.ncbi.nlm.nih.gov/pubmed/31197258
Comprehensive translocation and clonality detection in lymphoproliferative disorders by next generation sequencing
Wren D, Walker BA, Brüggemann M, Catherwood MA, Pott C, Stamatopoulos K, Langerak AW, Gonzalez D; on behalf of the EuroClonality-NGS consortium
Reference: Haematologica 2017;102:e57-e60.
Link: https://www.ncbi.nlm.nih.gov/pubmed/27846615
ARResT/Interrogate: an interactive immunoprofiler for IG/TR NGS data
Bystry V, Reigl T, Krejci A, Demko M, Hanakova B, Grioni A, Knecht H, Schlitt M, Dreger P, Sellner L, Herrmann D, Pingeon M, Boudjoghra M, Rijntjes J, Pott C, Langerak AW, Groenen PJTA, Davi F, Brüggemann M, Darzentas N; also on behalf of the EuroClonality-NGS consortium
Reference: Bioinformatics 2017;33:435-437.
Link: https://www.ncbi.nlm.nih.gov/pubmed/28172348
High throughput immunogenetics for clinical and research applications in immunohematology: potential and challenges
Langerak AW, Brüggemann M, Davi F, Darzentas N, Gonzalez D, Cazzaniga G, Giudicelli V, Lefranc MP, Giraud M, Macintyre E, Hummel M, Pott C, Groenen PJTA, Stamatopoulos K; on behalf of the EuroClonality-NGS consortium
Reference: J Immunol. 2017;198:3765-3774.
Link: https://www.ncbi.nlm.nih.gov/pubmed/28416603
Identification of IG-clonality status as a pre-treatment predictor for mortality in patients with immunodeficiency-associated Epstein-Barr virus-related lymphoproliferative disorders.
van der Velden VHJ, Nissen L, van Rijn M, Rijntjes J, de Haan A, Venkatraman L, Catherwood M, Liu H, El-Daly H, van de Laar L, Craenmehr MH, van Krieken JH, Stevens WB, Groenen PJTA.
Reference: Haematologica. 2015;100:e152-4.
The added value of immunoglobulin Kappa light chain gene (IGK) rearrangement analysis in suspected B-cell lymphomas: three illustrative cases.
Gameiro P, Sebastiăo M, Spetalen S, Gomes da Silva M, Cabeçadas
Reference: Journal of Hematopathology. 2012;5:45-56.
Link: https://link.springer.com/article/10.1007/s12308-011-0132-6
Multiple clonal Ig/TCR products: implications for interpretation of clonality findings.
Langerak AW and van Dongen JJM.
Reference: Journal of Hematopathology. 2012;5:35-43.
Link: https://link.springer.com/article/10.1007/s12308-011-0129-1
A comparative analysis of protocols for detection of T cell clonality in formalin-fixed, paraffin-embedded tissue—implications for practical use.
Bonzheim I, Fröhlich, Adam P, Colak S, Metzler G, Quintanilla-Martinez L, Fend F. A
Reference: Journal of Hematopathology. 2012;5:7-16.
Link: https://link.springer.com/article/10.1007/s12308-011-0128-2
Considerations for the use of formalin-fixed and paraffin-embedded tissue specimens for clonality analysis.
Lenze D, Müller HH, Hummel M.
Reference: Journal of Hematopathology. 2012; 5:27-34.
Link: https://link.springer.com/article/10.1007/s12308-012-0138-8
A practical approach to diagnostic Ig/TCR clonality evaluation in clinical pathology.
Groenen PJTA, van Raaij, A, van Altena MC, Rombout PM, van Krieken JHJM.
Reference: Journal of Hematopathology. 2012;5:17-25.
Link: https://link.springer.com/article/10.1007/s12308-011-0131-7
The EuroClonality website: information, education and support on clonality testing.
Rombout PDM, Diss TC, Hodges E, Hummel M, van Dongen JJM, Langerak AW, Groenen PJTA.
Reference: J. Hematopathol. 2012;5:99-103.
Link: https://link.springer.com/article/10.1007/s12308-011-0120-x
Clonality testing: teamwork by pathologist and molecular biologist.
van Krieken JH, Cabeçadas J, Groenen PJTA.
Reference: J. Hematopathol. 2012;5:3-5.
Link: https://link.springer.com/article/10.1007/s12308-012-0143-y
Clonality analysis in lymphoproliferative disease using the BIOMED-2 multiplex PCR protocols: experience from the EuroClonality group EQA scheme
Harris S, Brüggemann M, Groenen PJTA, Schuuring E, Langerak AW, Hodges E.
Reference: J. Hematopathol. 2012;5:91-98.
Link: https://link.springer.com/article/10.1007/s12308-012-0144-x
Clonal antigen receptor gene PCR products outside the expected size range.
Rothberg PG, Langerak AW, Verhaaf B, van Dongen JJM, Burack WJ, Johnson MD, Slate D, Laughlin TS, PayneK, Figueiredo L, Bandoh BN, Yan Q, Bacon CM, Wright P, Bench A, Du M-Q, Liu H.
Reference: Journal of Hematopathology. 2012;5:57-67.
Link: https://link.springer.com/article/10.1007/s12308-011-0135-3
Capillary electrophoresis single-strand conformation analysis (CE-SSCA) for clonality detection in lymphoproliferative disorders.
Gonzalez D, Rombout PDM, Sarasquete ME, Villarese P, Wren D, van Dongen JJM, Garcia-Sanz R, Macintyre E, Langerak AW, Groenen PJTA.
Reference: Journal of Hematopathology. 2012;5;83-89.
Link: https://link.springer.com/article/10.1007/s12308-011-0119-3
EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations
Langerak AW, Groenen PJ, Brüggemann M, Beldjord K, Bellan C, Bonello L, Boone E, Carter GI, Catherwood M, Davi F, Delfau-Larue MH, Diss T, Evans PA, Gameiro P, Garcia Sanz R, Gonzalez D, Grand D, Håkansson A, Hummel M, Liu H, Lombardia L, Macintyre E, Milner BJ, Montes-Moreno S, Schuuring E, Spaargaren M, Hodges E, van Dongen JJM
Reference: Leukemia. 2012;26:2159-71.
Link: https://pubmed.ncbi.nlm.nih.gov/22918122/
Pitfalls in TCR gene clonality testing: teaching cases
Groenen PJTA, Langerak AW, van Dongen JJM, van Krieken JH
Reference: J Hematop. 2008;1:97-109.
Link: https://pubmed.ncbi.nlm.nih.gov/19669208/
Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936
Evans PA, Pott C, Groenen PJTA, Salles G, Davi F, Berger F, Garcia JF, van Krieken JH, Pals S, Kluin P, Schuuring E, Spaargaren M, Boone E, González D, Martinez B, Villuendas R, Gameiro P, Diss TC, Mills K, Morgan GJ, Carter GI, Milner BJ, Pearson D, Hummel M, Jung W, Ott M, Canioni D, Beldjord K, Bastard C, Delfau-Larue MH, van Dongen JJM, Molina TJ, Cabeçadas J
Reference: Leukemia. 2007;21:207-14.
Link: https://pubmed.ncbi.nlm.nih.gov/17170731/
Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BMH4-CT98-3936
Langerak AW, Molina TJ, Lavender FL, Pearson D, Flohr T, Sambade C, Schuuring E, Al Saati T, van Dongen JJM, van Krieken JH
Reference: Leukemia. 2007;21:222-9.
Link: https://pubmed.ncbi.nlm.nih.gov/17170729/
Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936
Brüggemann M, White H, Gaulard P, Garcia-Sanz R, Gameiro P, Oeschger S, Jasani B, Ott M, Delsol G, Orfao A, Tiemann M, Herbst H, Langerak AW, Spaargaren M, Moreau E, Groenen PJTA, Sambade C, Foroni L, Carter GI, Hummel M, Bastard C, Davi F, Delfau-Larue MH, Kneba M, van Dongen JJM, Beldjord K, Molina TJ
Reference: Leukemia. 2007;21:215-21.
Link: https://pubmed.ncbi.nlm.nih.gov/17170730/
Immunoglobulin/T-cell receptor clonality diagnostics
Langerak AW, Groenen PJTA, Jm van Krieken JH, van Dongen JJM
Reference: Expert Opin Med Diagn. 2007;1:451-61.
Link: https://pubmed.ncbi.nlm.nih.gov/23496353/
Improved reliability of lymphoma diagnostics via PCR-based clonality testing: report of the BIOMED-2 Concerted Action BHM4-CT98-3936
van Krieken JH, Langerak AW, Macintyre E, Kneba M, Hodges E, Sanz RG, Morgan GJ, Parreira A, Molina TJ, Cabeçadas J, Gaulard P, Jasani B, Garcia JF, Ott M, Hannsmann ML, Berger F, Hummel M, Davi F, Brüggemann M, Lavender FL, Schuuring E, Evans PA, White H, Salles G, Groenen PJ, Gameiro P, Pott C, Dongen JJM
Reference: Leukemia. 2007;21:201-6.
Link: https://pubmed.ncbi.nlm.nih.gov/17170732/
Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes
Sandberg Y, Verhaaf B, van Gastel-Mol EJ, Wolvers-Tettero IL, de Vos J, Macleod RA, Noordzij JG, Dik WA, van Dongen JJM, Langerak AW.
Reference: Leukemia . 2007 Feb;21:230-7.
Wotherspoon criteria combined with B-cell clonality analysis by advanced polymerase chain reaction technology discriminates covert gastric marginal zone lymphoma from chronic gastritis.
Hummel M, Oeschger S, Barth TFE, Loddenkemper C, Cogliatti SB, Marx A, Wacker H-H, Feller AC, Bernd H-W, Hansmann M-L, Stein H, Möller P.
Reference: Gut/ 2006;55:782-787.
BIOMED-2 multiplex immunoglobulin/T-cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics
Sandberg Y, van Gastel-Mol EJ, Verhaaf B, Lam KH, van Dongen JJM, Langerak AW.
Reference: J Mol Diagn. 2005 Oct;7:495-503.
Validation of BIOMED-2 multiplex PCR tubes for detection of TCRB gene rearrangements in T-cell malignancies
Droese J, Langerak AW, Groenen PJ, Brüggemann M, Neumann P, Wolvers-Tettero IL, van Altena MC, Kneba M, van Dongen JJM.
Reference: Leukemia . 2004 Sep;18:1531-8.
TCRB gene rearrangements in childhood and adult precursor-B-ALL: frequency, applicability as MRD-PCR target, and stability between diagnosis and relapse
Van der Velden VHJ, Brüggemann M, Hoogeveen PG, De Bie M, Hart PG, Raff T, Pfeifer H, Luschen S, Szczepanski T, Van Wering ER, Kneba M, Van Dongen JJM.
Reference: Leukemia. 2004;18:1971-1980.
Rearranged T-cell receptor beta genes represent powerful targets for quantification of minimal residual disease in childhood and adult T-cell acute lymphoblastic leukemia
Brüggemann M, Van der Velden VHJ, Raff T, Droese J, Ritgen M, Pott C, Wijkhuijs AJ, Gokbuget N, Hoelzer D, Van Wering ER, Van Dongen JJM, Kneba M.
Reference: Leukemia. 2004;18:709-719.
Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936
van Dongen JJM, Langerak AW, Brüggemann M, Evans PA, Hummel M, Lavender FL, Delabesse E, Davi F, Schuuring E, García-Sanz R, van Krieken JH, Droese J, González D, Bastard C, White HE, Spaargaren M, González M, Parreira A, Smith JL, Morgan GJ, Kneba M, Macintyre E
Reference: Leukemia. 2003;17:2257-317.
Link: https://pubmed.ncbi.nlm.nih.gov/14671650/
Clonality analysis for antigen receptor genes: preliminary results from the Biomed-2 concerted action PL 96-3936
van Krieken JH, Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan GM, Kneba M, Macintyre E, van Dongen JJM
Reference: Hum Pathol. 2003;34:359-61.
Link: https://pubmed.ncbi.nlm.nih.gov/12733116/
Molecular immunoglobulin/T-cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol
Sandberg Y, Heule F, Lam K, Lugtenburg PJ, Wolvers-Tettero ILM, Van Dongen JJM, Langerak AW.
Reference: Journal of Hematopathology . 2003 Jun;88:659-670.
Polymerase chain reaction-based clonality diagnosis of lymphoproliferative disorders
Van Dongen JJM, Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan G, Kneba M, Van Krieken JHJM, Macintyre EA.
Reference: The Hematology Journal 2002;3S2:31-33
Clonality analysis in malignant lymphoma: the BIOMED-2 experience
Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan G, Kneba M, Macintyre E, Van Krieken JHJM, Van Dongen JJM.
Reference: Histopathol 2002;41S2:506-508.
Link: https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2559.41.s2.65.x