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EuroClonality Publications

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EuroClonality/BIOMED-2 primers
EQA
Educational workshop
Guidelines
EuroClonality-NGS Working Group
EuroClonality-NGS assays
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Clonality assessment
Capture-based NGS
MRD

Validation of the EuroClonality-NGS DNA capture panel as an integrated genomic tool for lymphoproliferative disorders

Stewart JP, Gazdova J, Darzentas N, Wren D, Proszek P, Fazio G, Songia S, Alcoceba M, Sarasquete ME, Villarese P, van der Klift MY, Heezen KC, McCafferty N, Pal K, Catherwood M, Kim CS, Srivastava S, Kroeze LI, Hodges E, Stamatopoulos K, Klapper W, Genuardi E, Ferrero S, van den Brand M, Cazzaniga G, Davi F, Sutton LA, Garcia-Sanz R, Groenen PJTA, Macintyre EA, Brüggemann M, Pott C, Langerak AW, Gonzalez D; EuroClonality-NGS Working Group.

Reference: Blood Adv. 2021;5:3188-3198

Link: https://pubmed.ncbi.nlm.nih.gov/34424321/

EuroClonality-NGS Working Group
Capture-based NGS
Clonality assessment
EuroClonality-NGS assays

Next-Generation Sequencing-Based Clonality Assessment of Ig Gene Rearrangements: A Multicenter Validation Study by EuroClonality-NGS

van den Brand M, Rijntjes J, Möbs M, Steinhilber J, van der Klift MY, Heezen KC, Kroeze LI, Reigl T, Porc J, Darzentas N, Luijks JACW, Scheijen B, Davi F, ElDaly H, Liu H, Anagnostopoulos I, Hummel M, Fend F, Langerak AW, Groenen PJTA; EuroClonality-NGS Working Group.

Reference: J Mol Diagn. 2021;23:1105-1115

Link: https://pubmed.ncbi.nlm.nih.gov/34186174/

Clonality assessment
EuroClonality-NGS Working Group
EuroClonality-NGS assays

Potential and pitfalls of whole transcriptome-based immunogenetic marker identification in acute lymphoblastic leukemia; a EuroMRD and EuroClonality-NGS Working Group study.

van der Velden VHJ, Brüggemann M, Cazzaniga G, Scheijen B, Tops B, Trka J, Pal K, Hänzelmann S, Fazio G, Songia S, Langerak AW, Darzentas N; EuroMRD; EuroClonality-NGS Working Group.

Reference: Leukemia. 2021;35:924-928

Link: https://pubmed.ncbi.nlm.nih.gov/33608635/

EuroClonality-NGS assays
Bioinformatics
EuroClonality-NGS Working Group
MRD

Evaluation of a worldwide EQA scheme for complex clonality analysis of clinical lymphoproliferative cases demonstrates a learning effect

Keppens C, Boone E, Gameiro P, Tack V, Moreau E, Hodges E, Evans P, Brüggemann M, Carter I, Lenze D, Sarasquete ME, Möbs M, Liu H, Dequeker EMC, Groenen PJTA

Reference: Virchows Arch. 2021;479:365-376

Link: https://pubmed.ncbi.nlm.nih.gov/33686511/

Clonality assessment
EuroClonality/BIOMED-2 primers
EQA

Next-generation sequencing technology to identify minimal residual disease in lymphoid malignancies

Kotrova M, Darzentas N, Pott C, Brüggemann M; EuroClonality-NGS Working Group

Reference: Methods Mol Biol. 2021;2185:95-111

Link: https://pubmed.ncbi.nlm.nih.gov/33165845/

EuroClonality-NGS Working Group
MRD
EuroClonality-NGS assays

Immunoglobulin gene analysis in chronic lymphocytic leukemia in the era of next generation sequencing

Davi F, Langerak AW, de Septenville AL, Kolijn PM, Hengeveld PJ, Chatzidimitriou A, Bonfiglio S, Sutton LA, Rosenquist R, Ghia P, Stamatopoulos K; ERIC, the European Research Initiative on CLL, and the EuroClonality-NGS Working Group

Reference: Leukemia. 2020;34:2545-2551

Link: https://pubmed.ncbi.nlm.nih.gov/32561841/

EuroClonality-NGS Working Group

Memento for interprofessional learning

Groenen PJTA, Langerak AW, Fend F, van Krieken JHJM

Reference: Virchows Arch. 2020;477:755-756

Link: https://pubmed.ncbi.nlm.nih.gov/32270299/

Educational workshop
EuroClonality/BIOMED-2 primers

A new and simple TRG multiplex PCR assay for assessment of T-cell clonality: A comparative study from the EuroClonality Consortium

Armand M, Derrieux C, Beldjord K, Wabeke T, Lenze D, Boone E, Bruggemann M, Evans PAS, Gameiro P, Hummel M, Villarese P, Groenen PJTA, Langerak AW, Macintyre EA, Davi F

Reference: Hemasphere. 2019;3:e255

Link: https://pubmed.ncbi.nlm.nih.gov/31723840/

Clonality assessment
EuroClonality/BIOMED-2 primers

Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study

Brüggemann M, Kotrová M, Knecht H, Bartram J, Boudjogrha M, Bystry V, Fazio G, Froňková E, Giraud M, Grioni A, Hancock J, Herrmann D, Jiménez C, Krejci A, Moppett J, Reigl T, Salson M, Scheijen B, Schwarz M, Songia S, Svaton M, van Dongen JJM, Villarese P, Wakeman S, Wright G, Cazzaniga G, Davi F, García-Sanz R, Gonzalez D, Groenen PJTA, Hummel M, Macintyre EA, Stamatopoulos K, Pott C, Trka J, Darzentas N, Langerak AW; EuroClonality-NGS working group

Reference: Leukemia. 2019;33:2241-2253

Link: https://www.ncbi.nlm.nih.gov/pubmed/31243313

MRD
EuroClonality-NGS Working Group
EuroClonality-NGS assays

Quality control and quantification in IG/TR next-generation sequencing marker identification: protocols and bioinformatic functionalities by EuroClonality-NGS

Knecht H, Reigl T, Kotrová M, Appelt F, Stewart P, Bystry V, Krejci A, Grioni A, Pal K, Stranska K, Plevova K, Rijntjes J, Songia S, Svatoň M, Froňková E, Bartram J, Scheijen B, Herrmann D, García-Sanz R, Hancock J, Moppett J, van Dongen JJM, Cazzaniga G, Davi F, Groenen PJTA, Hummel M, Macintyre EA, Stamatopoulos K, Trka J, Langerak AW, Gonzalez D, Pott C, Brüggemann M, Darzentas N; EuroClonality-NGS Working Group

Reference: Leukemia 2019;33:2254-2265 (2019 Jun 21)

Link: https://www.ncbi.nlm.nih.gov/pubmed/31227779

Bioinformatics
EuroClonality-NGS Working Group
EuroClonality-NGS assays

Next-generation sequencing of immunoglobulin gene rearrangements for clonality assessment: a technical feasibility study by EuroClonality-NGS

Scheijen B, Meijers RWJ, Rijntjes J, van der Klift MY, Möbs M, Steinhilber J, Reigl T, van den Brand M, Kotrová M, Ritter JM, Catherwood MA, Stamatopoulos K, Brüggemann M, Davi F, Darzentas N, Pott C, Fend F, Hummel M, Langerak AW, Groenen PJTA; EuroClonality-NGS Working Group

Reference: Leukemia 2019;33:2227-2240

Link: https://www.ncbi.nlm.nih.gov/pubmed/31197258

EuroClonality-NGS Working Group
EuroClonality-NGS assays
Clonality assessment

PCR GeneScan and heteroduplex analysis of rearranged immunoglobulin or T-cell receptor genes for clonality diagnostics in suspect lymphoproliferations

Boone E, Heezen KC, Groenen PJTA, Langerak AW; EuroClonality Consortium

Reference: Methods Mol Biol. 2019;1956:77-103

Link: https://pubmed.ncbi.nlm.nih.gov/30779031/

EuroClonality/BIOMED-2 primers
Clonality assessment

High-throughput immunogenetics for clinical and research applications in immuno-hematology: potential and challenges

Langerak AW, Brüggemann M, Davi F, Darzentas N, Gonzalez D, Cazzaniga G, Giudicelli V, Lefranc MP, Giraud M, Macintyre EA, Hummel M, Pott C, Groenen PJTA, Stamatopoulos K; on behalf of the EuroClonality-NGS consortium

Reference: J Immunol 2017;198:3765-3774

Link: https://www.ncbi.nlm.nih.gov/pubmed/28416603

EuroClonality-NGS Working Group

Comprehensive translocation and clonality detection in lymphoproliferative disorders by next generation sequencing

Wren D, Walker BA, Brüggemann M, Catherwood MA, Pott C, Stamatopoulos K, Langerak AW, Gonzalez D; on behalf of the EuroClonality-NGS consortium

Reference: Haematologica 2017;102:e57-e60

Link: https://www.ncbi.nlm.nih.gov/pubmed/27846615

EuroClonality-NGS Working Group

ARResT/Interrogate: an interactive immunoprofiler for IG/TR NGS data

Bystry V, Reigl T, Krejci A, Demko M, Hanakova B, Grioni A, Knecht H, Schlitt M, Dreger P, Sellner L, Herrmann D, Pingeon M, Boudjoghra M, Rijntjes J, Pott C, Langerak AW, Groenen PJTA, Davi F, Brüggemann M, Darzentas N; also on behalf of the EuroClonality-NGS consortium

Reference: Bioinformatics 2017;33:435-437

Link: https://www.ncbi.nlm.nih.gov/pubmed/28172348

EuroClonality-NGS Working Group
Bioinformatics

EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations

Langerak AW, Groenen PJ, Brüggemann M, Beldjord K, Bellan C, Bonello L, Boone E, Carter GI, Catherwood M, Davi F, Delfau-Larue MH, Diss T, Evans PA, Gameiro P, Garcia Sanz R, Gonzalez D, Grand D, Håkansson A, Hummel M, Liu H, Lombardia L, Macintyre EA, Milner BJ, Montes-Moreno S, Schuuring E, Spaargaren M, Hodges E, van Dongen JJ

Reference: Leukemia. 2012;26:2159-71

Link: https://pubmed.ncbi.nlm.nih.gov/22918122/

EuroClonality/BIOMED-2 primers
Clonality assessment
Guidelines

Pitfalls in TCR gene clonality testing: teaching cases

Groenen PJ, Langerak AW, van Dongen JJ, van Krieken JH

Reference: J Hematop. 2008;1:97-109

Link: https://pubmed.ncbi.nlm.nih.gov/19669208/

EuroClonality/BIOMED-2 primers
Clonality assessment
Educational workshop

Immunoglobulin/T-cell receptor clonality diagnostics

Langerak AW, Groenen PJ, Jm van Krieken JH, van Dongen JJ

Reference: Expert Opin Med Diagn. 2007;1:451-61

Link: https://pubmed.ncbi.nlm.nih.gov/23496353/

Clonality assessment

Improved reliability of lymphoma diagnostics via PCR-based clonality testing: report of the BIOMED-2 Concerted Action BHM4-CT98-3936

van Krieken JH, Langerak AW, Macintyre EA, Kneba M, Hodges E, Sanz RG, Morgan GJ, Parreira A, Molina TJ, Cabeçadas J, Gaulard P, Jasani B, Garcia JF, Ott M, Hannsmann ML, Berger F, Hummel M, Davi F, Brüggemann M, Lavender FL, Schuuring E, Evans PA, White H, Salles G, Groenen PJ, Gameiro P, Pott Ch, Dongen JJ

Reference: Leukemia. 2007;21:201-6

Link: https://pubmed.ncbi.nlm.nih.gov/17170732/

Clonality assessment
EuroClonality/BIOMED-2 primers

Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

Evans PA, Pott Ch, Groenen PJ, Salles G, Davi F, Berger F, Garcia JF, van Krieken JH, Pals S, Kluin P, Schuuring E, Spaargaren M, Boone E, González D, Martinez B, Villuendas R, Gameiro P, Diss TC, Mills K, Morgan GJ, Carter GI, Milner BJ, Pearson D, Hummel M, Jung W, Ott M, Canioni D, Beldjord K, Bastard C, Delfau-Larue MH, van Dongen JJ, Molina TJ, Cabeçadas J

Reference: Leukemia. 2007;21:207-14

Link: https://pubmed.ncbi.nlm.nih.gov/17170731/

Clonality assessment
EuroClonality/BIOMED-2 primers

Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936

Brüggemann M, White H, Gaulard P, Garcia-Sanz R, Gameiro P, Oeschger S, Jasani B, Ott M, Delsol G, Orfao A, Tiemann M, Herbst H, Langerak AW, Spaargaren M, Moreau E, Groenen PJ, Sambade C, Foroni L, Carter GI, Hummel M, Bastard C, Davi F, Delfau-Larue MH, Kneba M, van Dongen JJ, Beldjord K, Molina TJ

Reference: Leukemia. 2007;21:215-21

Link: https://pubmed.ncbi.nlm.nih.gov/17170730/

Clonality assessment
EuroClonality/BIOMED-2 primers

Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BMH4-CT98-3936

Langerak AW, Molina TJ, Lavender FL, Pearson D, Flohr T, Sambade C, Schuuring E, Al Saati T, van Dongen JJ, van Krieken JH

Reference: Leukemia. 2007;21:222-9

Link: https://pubmed.ncbi.nlm.nih.gov/17170729/

Clonality assessment
EuroClonality/BIOMED-2 primers

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936

van Dongen JJ, Langerak AW, Brüggemann M, Evans PA, Hummel M, Lavender FL, Delabesse E, Davi F, Schuuring E, García-Sanz R, van Krieken JH, Droese J, González D, Bastard C, White HE, Spaargaren M, González M, Parreira A, Smith JL, Morgan GJ, Kneba M, Macintyre EA

Reference: Leukemia. 2003;17:2257-317

Link: https://pubmed.ncbi.nlm.nih.gov/14671650/

EuroClonality/BIOMED-2 primers
Clonality assessment

Clonality analysis for antigen receptor genes: preliminary results from the Biomed-2 concerted action PL 96-3936

van Krieken JH, Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan GM, Kneba M, Macintyre EA, van Dongen JJ

Reference: Hum Pathol. 2003;34:359-61

Link: https://pubmed.ncbi.nlm.nih.gov/12733116/

EuroClonality/BIOMED-2 primers
Clonality assessment