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EuroClonality Publications

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EuroClonality/BIOMED-2 primers
EQA
Educational workshop
Guidelines
EuroClonality-NGS Working Group
EuroClonality-NGS assays
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Clonality assessment
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MRD
Repertoire studies
SHM
Methods Mol Biol book on Immunogenetics
Amplicon-based NGS

EuroClonality-NGS Recommendations for Evaluation of B-Cell Clonality Analysis by Next-Generation Sequencing: A Structured Approach with the DEPART Algorithm

van den Brand M, Möbs M, Otto F, Kroeze LI, Gonzalez de Castro D, Stamatopoulos K, Davi F, Bravetti C, Kolijn PM, Vlachonikola E, Stewart JP, Pott C, Hummel M, Darzentas N, Langerak AW, Fend F, Groenen PJTA.

Reference: J Mol Diagn. 2023;25:729-739.

Link: https://pubmed.ncbi.nlm.nih.gov/37467928/

Guidelines
Clonality assessment
EuroClonality-NGS assays
Amplicon-based NGS
Bioinformatics
EuroClonality-NGS Working Group

Clonotype definitions for immunogenetic studies: proposals from the EuroClonality NGS Working Group

Sofou E, Vlachonikola E, Zaragoza-Infante L, Brüggemann M, Darzentas N, Groenen PJTA, Hummel M, Macintyre EA, Psomopoulos F, Davi F, Langerak AW, Stamatopoulos K.

Reference: Leukemia. 2023 Aug 37;1750-1752.

Link: https://pubmed.ncbi.nlm.nih.gov/37391484/

MRD
Guidelines
EuroClonality-NGS Working Group
Clonality assessment
Repertoire studies

A novel next-generation sequencing capture-based strategy to report somatic hypermutation status using genomic regions downstream to immunoglobulin rearrangements

McCafferty N, Stewart JP, Darzentas N, Gazdova J, Catherwood M, Stamatopoulos K, Langerak AW, Gonzalez D

Reference: Haematologica . 2023 May 1;108:1313-1321.

Link: https://pubmed.ncbi.nlm.nih.gov/36579446/

Capture-based NGS
EuroClonality-NGS Working Group
SHM
EuroClonality-NGS assays

Read the clonotype: Next-generation sequencing-based lymphocyte clonality analysis and perspectives for application in pathology

Groenen PJTA, van den Brand M, Kroeze LI, Amir AL, Hebeda KM.

Reference: Front Oncol. 2023 Feb 8;13:1107171.

Link: https://pubmed.ncbi.nlm.nih.gov/36845702/

Clonality assessment
EuroClonality-NGS assays
Amplicon-based NGS

The Advent of Precision Immunology: Immunogenetics at the Center of Immune Cell Analysis in Health and Disease

Langerak AW

Reference: Methods Mol Biol. 2022;2453:1-5.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_1

Methods Mol Biol book on Immunogenetics
EuroClonality-NGS Working Group

Next-generation sequencing-based clonality detection of immunoglobulin gene rearrangements in B-cell lymphoma

van Bladel DAG, van der Last-Kempkes JLM, Scheijen B, Groenen PJTA; EuroClonality Consortium.

Reference: Methods Mol Biol. 2022;2453:7-42.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_2

Methods Mol Biol book on Immunogenetics
Amplicon-based NGS
EuroClonality-NGS Working Group
Clonality assessment
EuroClonality-NGS assays

One-Step Next-Generation Sequencing of Immunoglobulin and T-Cell Receptor Gene Recombinations for MRD Marker Identification in Acute Lymphoblastic Leukemia

Villarese P, Abdo C, Bertrand M, Thonier F, Giraud M, Salson M, Macintyre E

Reference: Methods Mol Biol. 2022;2453:43-59

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_3

Amplicon-based NGS
MRD
EuroClonality-NGS Working Group
Methods Mol Biol book on Immunogenetics

Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq

van der Velden VHJ, Bastian L, Brüggemann M, Hartmann AM, Darzentas N.

Reference: Methods Mol Biol. 2022;2453:61-77

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_4

Methods Mol Biol book on Immunogenetics
EuroClonality-NGS Working Group
MRD
Bioinformatics

Quality control for IG /TR marker identification and MRD analysis

Fronkova E, Svaton M, Trka J

Reference: Methods Mol Biol. 2022;2453:91-99.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_6

EuroClonality-NGS Working Group
MRD
Methods Mol Biol book on Immunogenetics

cfDNA-Based NGS IG Analysis in Lymphoma

Pott C, Kotrova M, Darzentas N, Brüggemann M, Khouja M; EuroClonality-NGS Working Group

Reference: Methods Mol Biol. 2022;2453:101-117.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_7

MRD
EuroClonality-NGS Working Group
EuroClonality-NGS assays
Clonality assessment
Methods Mol Biol book on Immunogenetics
Amplicon-based NGS

Targeted locus amplification as marker screening approach to detect immunoglobulin (IG) translocations in B-cell non-hodgkin lymphomas

Genuardi E, Alessandria B, Civita AM, Ferrero S.

Reference: Methods Mol Biol. 2022;2453:119-132.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_8

EuroClonality-NGS Working Group
Methods Mol Biol book on Immunogenetics
MRD

Immunoglobulin/T cell receptor capture strategy for comprehensive immunogenetics

Stewart JP, Gazdova J, Srivastava S, Revolta J, Harewood L, Maurya M, Darzentas N, Gonzalez D

Reference: Methods Mol Biol. 2022;2453:133-152.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_9

Capture-based NGS
EuroClonality-NGS assays
EuroClonality-NGS Working Group
Methods Mol Biol book on Immunogenetics

Immunoglobulin gene mutational status assessment by next generation sequencing in chronic lymphocytic Leukemia

Langlois de Septenville A, Boudjoghra M, Bravetti C, Armand M, Salson M, Giraud M, Davi F

Reference: Methods Mol Biol. 2022;2453:153-167.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_10

Methods Mol Biol book on Immunogenetics
SHM
EuroClonality-NGS assays
Repertoire studies
EuroClonality-NGS Working Group

ARResT/Interrogate Immunoprofiling platform: concepts, workflows, and insights

Darzentas N

Reference: Methods Mol Biol. 2022;2453:571-584.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_26

EuroClonality-NGS Working Group
Bioinformatics
Methods Mol Biol book on Immunogenetics

Purpose-built immunoinformatics for BcR IG/TR repertoire data analysis

Galigalidou C, Zaragoza-Infante L, Chatzidimitriou A, Stamatopoulos K, Psomopoulos F, Agathangelidis A

Reference: Methods Mol Biol. 2022;2453:585-603.

Link: https://link.springer.com/protocol/10.1007/978-1-0716-2115-8_27

EuroClonality-NGS Working Group
Repertoire studies
Bioinformatics
Methods Mol Biol book on Immunogenetics

Next-generation sequencing technology to identify minimal residual disease in lymphoid malignancies

Kotrova M, Darzentas N, Pott C, Brüggemann M; EuroClonality-NGS Working Group

Reference: Methods Mol Biol. 2021;2185:95-111.

Link: https://pubmed.ncbi.nlm.nih.gov/33165845/

MRD
EuroClonality-NGS Working Group
EuroClonality-NGS assays

Evaluation of a worldwide EQA scheme for complex clonality analysis of clinical lymphoproliferative cases demonstrates a learning effect

Keppens C, Boone E, Gameiro P, Tack V, Moreau E, Hodges E, Evans P, Brüggemann M, Carter I, Lenze D, Sarasquete ME, Möbs M, Liu H, Dequeker EMC, Groenen PJTA

Reference: Virchows Arch. 2021;479:365-376.

Link: https://pubmed.ncbi.nlm.nih.gov/33686511/

Clonality assessment
EuroClonality/BIOMED-2 primers
EQA

Next-generation sequencing-based clonality assessment of Ig Gene Rearrangements: A multicenter validation study by EuroClonality-NGS

van den Brand M, Rijntjes J, Möbs M, Steinhilber J, van der Klift MY, Heezen KC, Kroeze LI, Reigl T, Porc J, Darzentas N, Luijks JACW, Scheijen B, Davi F, ElDaly H, Liu H, Anagnostopoulos I, Hummel M, Fend F, Langerak AW, Groenen PJTA; EuroClonality-NGS Working Group.

Reference: J Mol Diagn. 2021;23:1105-1115.

Link: https://pubmed.ncbi.nlm.nih.gov/34186174/

Amplicon-based NGS
EuroClonality-NGS assays
Clonality assessment
EuroClonality-NGS Working Group

Potential and pitfalls of whole transcriptome-based immunogenetic marker identification in acute lymphoblastic leukemia; a EuroMRD and EuroClonality-NGS Working Group study.

van der Velden VHJ, Brüggemann M, Cazzaniga G, Scheijen B, Tops B, Trka J, Pal K, Hänzelmann S, Fazio G, Songia S, Langerak AW, Darzentas N; EuroMRD; EuroClonality-NGS Working Group.

Reference: Leukemia. 2021;35:924-928.

Link: https://pubmed.ncbi.nlm.nih.gov/33608635/

EuroClonality-NGS assays
Bioinformatics
EuroClonality-NGS Working Group
MRD

Validation of the EuroClonality-NGS DNA capture panel as an integrated genomic tool for lymphoproliferative disorders

Stewart JP, Gazdova J, Darzentas N, Wren D, Proszek P, Fazio G, Songia S, Alcoceba M, Sarasquete ME, Villarese P, van der Klift MY, Heezen KC, McCafferty N, Pal K, Catherwood M, Kim CS, Srivastava S, Kroeze LI, Hodges E, Stamatopoulos K, Klapper W, Genuardi E, Ferrero S, van den Brand M, Cazzaniga G, Davi F, Sutton LA, Garcia-Sanz R, Groenen PJTA, Macintyre E, Brüggemann M, Pott C, Langerak AW, Gonzalez D; EuroClonality-NGS Working Group.

Reference: Blood Adv. 2021;5:3188-3198.

Link: https://pubmed.ncbi.nlm.nih.gov/34424321/

EuroClonality-NGS Working Group
Clonality assessment
EuroClonality-NGS assays
Capture-based NGS

Immunoglobulin gene analysis in chronic lymphocytic leukemia in the era of next generation sequencing

Davi F, Langerak AW, de Septenville AL, Kolijn PM, Hengeveld PJ, Chatzidimitriou A, Bonfiglio S, Sutton LA, Rosenquist R, Ghia P, Stamatopoulos K; ERIC, the European Research Initiative on CLL, and the EuroClonality-NGS Working Group

Reference: Leukemia. 2020;34:2545-2551.

Link: https://pubmed.ncbi.nlm.nih.gov/32561841/

EuroClonality-NGS Working Group

Automation of amplicon-based library preparation for next-generation sequencing by centrifugal microfluidics

Hess JF, Kotrová M, Calabrese S, Darzentas N, Hutzenlaub T, Zengerle R, Brüggemann M, Paust N

Reference: Anal Chem. 2020;92:12833-12841

Link: https://pubmed.ncbi.nlm.nih.gov/32842730/

Memento for interprofessional learning

Groenen PJTA, Langerak AW, Fend F, van Krieken JHJM

Reference: Virchows Arch. 2020;477:755-756.

Link: https://pubmed.ncbi.nlm.nih.gov/32270299/

EuroClonality/BIOMED-2 primers
Educational workshop

PCR GeneScan and heteroduplex analysis of rearranged immunoglobulin or T-cell receptor genes for clonality diagnostics in suspect lymphoproliferations

Boone E, Heezen KC, Groenen PJTA, Langerak AW; EuroClonality Consortium

Reference: Methods Mol Biol. 2019;1956:77-103.

Link: https://pubmed.ncbi.nlm.nih.gov/30779031/

EuroClonality/BIOMED-2 primers
Clonality assessment

Next-generation sequencing of immunoglobulin gene rearrangements for clonality assessment: a technical feasibility study by EuroClonality-NGS

Scheijen B, Meijers RWJ, Rijntjes J, van der Klift MY, Möbs M, Steinhilber J, Reigl T, van den Brand M, Kotrová M, Ritter JM, Catherwood MA, Stamatopoulos K, Brüggemann M, Davi F, Darzentas N, Pott C, Fend F, Hummel M, Langerak AW, Groenen PJTA; EuroClonality-NGS Working Group

Reference: Leukemia 2019;33:2227-2240.

Link: https://www.ncbi.nlm.nih.gov/pubmed/31197258

EuroClonality-NGS Working Group
EuroClonality-NGS assays
Amplicon-based NGS
Clonality assessment

A new and simple TRG multiplex PCR assay for assessment of T-cell clonality: A comparative study from the EuroClonality Consortium

Armand M, Derrieux C, Beldjord K, Wabeke T, Lenze D, Boone E, Brüggemann M, Evans PAS, Gameiro P, Hummel M, Villarese P, Groenen PJTA, Langerak AW, Macintyre E, Davi F

Reference: Hemasphere. 2019;3:e255.

Link: https://pubmed.ncbi.nlm.nih.gov/31723840/

EuroClonality/BIOMED-2 primers
Clonality assessment

Quality control and quantification in IG/TR next-generation sequencing marker identification: protocols and bioinformatic functionalities by EuroClonality-NGS

Knecht H, Reigl T, Kotrová M, Appelt F, Stewart P, Bystry V, Krejci A, Grioni A, Pal K, Stranska K, Plevova K, Rijntjes J, Songia S, Svatoň M, Froňková E, Bartram J, Scheijen B, Herrmann D, García-Sanz R, Hancock J, Moppett J, van Dongen JJM, Cazzaniga G, Davi F, Groenen PJTA, Hummel M, Macintyre E, Stamatopoulos K, Trka J, Langerak AW, Gonzalez D, Pott C, Brüggemann M, Darzentas N; EuroClonality-NGS Working Group

Reference: Leukemia. 2019;33:2254-2265 (2019 Jun 21)

Link: https://www.ncbi.nlm.nih.gov/pubmed/31227779

Amplicon-based NGS
Bioinformatics
EuroClonality-NGS Working Group
EuroClonality-NGS assays

Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study

Brüggemann M, Kotrová M, Knecht H, Bartram J, Boudjogrha M, Bystry V, Fazio G, Froňková E, Giraud M, Grioni A, Hancock J, Herrmann D, Jiménez C, Krejci A, Moppett J, Reigl T, Salson M, Scheijen B, Schwarz M, Songia S, Svaton M, van Dongen JJM, Villarese P, Wakeman S, Wright G, Cazzaniga G, Davi F, García-Sanz R, Gonzalez D, Groenen PJTA, Hummel M, Macintyre E, Stamatopoulos K, Pott C, Trka J, Darzentas N, Langerak AW; EuroClonality-NGS working group

Reference: Leukemia. 2019;33:2241-2253.

Link: https://www.ncbi.nlm.nih.gov/pubmed/31243313

MRD
EuroClonality-NGS assays
Amplicon-based NGS
EuroClonality-NGS Working Group

Comprehensive translocation and clonality detection in lymphoproliferative disorders by next generation sequencing

Wren D, Walker BA, Brüggemann M, Catherwood MA, Pott C, Stamatopoulos K, Langerak AW, Gonzalez D; on behalf of the EuroClonality-NGS consortium

Reference: Haematologica 2017;102:e57-e60.

Link: https://www.ncbi.nlm.nih.gov/pubmed/27846615

EuroClonality-NGS Working Group

ARResT/Interrogate: an interactive immunoprofiler for IG/TR NGS data

Bystry V, Reigl T, Krejci A, Demko M, Hanakova B, Grioni A, Knecht H, Schlitt M, Dreger P, Sellner L, Herrmann D, Pingeon M, Boudjoghra M, Rijntjes J, Pott C, Langerak AW, Groenen PJTA, Davi F, Brüggemann M, Darzentas N; also on behalf of the EuroClonality-NGS consortium

Reference: Bioinformatics 2017;33:435-437.

Link: https://www.ncbi.nlm.nih.gov/pubmed/28172348

Bioinformatics
EuroClonality-NGS Working Group

High throughput immunogenetics for clinical and research applications in immunohematology: potential and challenges

Langerak AW, Brüggemann M, Davi F, Darzentas N, Gonzalez D, Cazzaniga G, Giudicelli V, Lefranc MP, Giraud M, Macintyre E, Hummel M, Pott C, Groenen PJTA, Stamatopoulos K; on behalf of the EuroClonality-NGS consortium

Reference: J Immunol. 2017;198:3765-3774.

Link: https://www.ncbi.nlm.nih.gov/pubmed/28416603

EuroClonality-NGS Working Group

Identification of IG-clonality status as a pre-treatment predictor for mortality in patients with immunodeficiency-associated Epstein-Barr virus-related lymphoproliferative disorders.

van der Velden VHJ, Nissen L, van Rijn M, Rijntjes J, de Haan A, Venkatraman L, Catherwood M, Liu H, El-Daly H, van de Laar L, Craenmehr MH, van Krieken JH, Stevens WB, Groenen PJTA.

Reference: Haematologica. 2015;100:e152-4.

Link: https://pubmed.ncbi.nlm.nih.gov/25527569/

EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations

Langerak AW, Groenen PJ, Brüggemann M, Beldjord K, Bellan C, Bonello L, Boone E, Carter GI, Catherwood M, Davi F, Delfau-Larue MH, Diss T, Evans PA, Gameiro P, Garcia Sanz R, Gonzalez D, Grand D, Håkansson A, Hummel M, Liu H, Lombardia L, Macintyre E, Milner BJ, Montes-Moreno S, Schuuring E, Spaargaren M, Hodges E, van Dongen JJM

Reference: Leukemia. 2012;26:2159-71.

Link: https://pubmed.ncbi.nlm.nih.gov/22918122/

EuroClonality/BIOMED-2 primers
Clonality assessment
Guidelines

Capillary electrophoresis single-strand conformation analysis (CE-SSCA) for clonality detection in lymphoproliferative disorders.

Gonzalez D, Rombout PDM, Sarasquete ME, Villarese P, Wren D, van Dongen JJM, Garcia-Sanz R, Macintyre E, Langerak AW, Groenen PJTA.

Reference: Journal of Hematopathology. 2012;5;83-89.

Link: https://link.springer.com/article/10.1007/s12308-011-0119-3

Considerations for the use of formalin-fixed and paraffin-embedded tissue specimens for clonality analysis.

Lenze D, Müller HH, Hummel M.

Reference: Journal of Hematopathology. 2012; 5:27-34.

Link: https://link.springer.com/article/10.1007/s12308-012-0138-8

A comparative analysis of protocols for detection of T cell clonality in formalin-fixed, paraffin-embedded tissue—implications for practical use.

Bonzheim I, Fröhlich, Adam P, Colak S, Metzler G, Quintanilla-Martinez L, Fend F. A

Reference: Journal of Hematopathology. 2012;5:7-16.

Link: https://link.springer.com/article/10.1007/s12308-011-0128-2

The added value of immunoglobulin Kappa light chain gene (IGK) rearrangement analysis in suspected B-cell lymphomas: three illustrative cases.

Gameiro P, Sebastiăo M, Spetalen S, Gomes da Silva M, Cabeçadas

Reference: Journal of Hematopathology. 2012;5:45-56.

Link: https://link.springer.com/article/10.1007/s12308-011-0132-6

Multiple clonal Ig/TCR products: implications for interpretation of clonality findings.

Langerak AW and van Dongen JJM.

Reference: Journal of Hematopathology. 2012;5:35-43.

Link: https://link.springer.com/article/10.1007/s12308-011-0129-1

A practical approach to diagnostic Ig/TCR clonality evaluation in clinical pathology.

Groenen PJTA, van Raaij, A, van Altena MC, Rombout PM, van Krieken JHJM.

Reference: Journal of Hematopathology. 2012;5:17-25.

Link: https://link.springer.com/article/10.1007/s12308-011-0131-7

The EuroClonality website: information, education and support on clonality testing.

Rombout PDM, Diss TC, Hodges E, Hummel M, van Dongen JJM, Langerak AW, Groenen PJTA.

Reference: J. Hematopathol. 2012;5:99-103.

Link: https://link.springer.com/article/10.1007/s12308-011-0120-x

Clonality testing: teamwork by pathologist and molecular biologist.

van Krieken JH, Cabeçadas J, Groenen PJTA.

Reference: J. Hematopathol. 2012;5:3-5.

Link: https://link.springer.com/article/10.1007/s12308-012-0143-y

Clonality analysis in lymphoproliferative disease using the BIOMED-2 multiplex PCR protocols: experience from the EuroClonality group EQA scheme

Harris S, Brüggemann M, Groenen PJTA, Schuuring E, Langerak AW, Hodges E.

Reference: J. Hematopathol. 2012;5:91-98.

Link: https://link.springer.com/article/10.1007/s12308-012-0144-x

EQA
EuroClonality/BIOMED-2 primers

Clonal antigen receptor gene PCR products outside the expected size range.

Rothberg PG, Langerak AW, Verhaaf B, van Dongen JJM, Burack WJ, Johnson MD, Slate D, Laughlin TS, PayneK, Figueiredo L, Bandoh BN, Yan Q, Bacon CM, Wright P, Bench A, Du M-Q, Liu H.

Reference: Journal of Hematopathology. 2012;5:57-67.

Link: https://link.springer.com/article/10.1007/s12308-011-0135-3

Pitfalls in TCR gene clonality testing: teaching cases

Groenen PJTA, Langerak AW, van Dongen JJM, van Krieken JH

Reference: J Hematop. 2008;1:97-109.

Link: https://pubmed.ncbi.nlm.nih.gov/19669208/

Clonality assessment
EuroClonality/BIOMED-2 primers
Educational workshop

Immunoglobulin/T-cell receptor clonality diagnostics

Langerak AW, Groenen PJTA, Jm van Krieken JH, van Dongen JJM

Reference: Expert Opin Med Diagn. 2007;1:451-61.

Link: https://pubmed.ncbi.nlm.nih.gov/23496353/

Clonality assessment

Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936

Brüggemann M, White H, Gaulard P, Garcia-Sanz R, Gameiro P, Oeschger S, Jasani B, Ott M, Delsol G, Orfao A, Tiemann M, Herbst H, Langerak AW, Spaargaren M, Moreau E, Groenen PJTA, Sambade C, Foroni L, Carter GI, Hummel M, Bastard C, Davi F, Delfau-Larue MH, Kneba M, van Dongen JJM, Beldjord K, Molina TJ

Reference: Leukemia. 2007;21:215-21.

Link: https://pubmed.ncbi.nlm.nih.gov/17170730/

Clonality assessment
EuroClonality/BIOMED-2 primers

Polymerase chain reaction-based clonality testing in tissue samples with reactive lymphoproliferations: usefulness and pitfalls. A report of the BIOMED-2 Concerted Action BMH4-CT98-3936

Langerak AW, Molina TJ, Lavender FL, Pearson D, Flohr T, Sambade C, Schuuring E, Al Saati T, van Dongen JJM, van Krieken JH

Reference: Leukemia. 2007;21:222-9.

Link: https://pubmed.ncbi.nlm.nih.gov/17170729/

Clonality assessment
EuroClonality/BIOMED-2 primers

Significantly improved PCR-based clonality testing in B-cell malignancies by use of multiple immunoglobulin gene targets. Report of the BIOMED-2 Concerted Action BHM4-CT98-3936

Evans PA, Pott C, Groenen PJTA, Salles G, Davi F, Berger F, Garcia JF, van Krieken JH, Pals S, Kluin P, Schuuring E, Spaargaren M, Boone E, González D, Martinez B, Villuendas R, Gameiro P, Diss TC, Mills K, Morgan GJ, Carter GI, Milner BJ, Pearson D, Hummel M, Jung W, Ott M, Canioni D, Beldjord K, Bastard C, Delfau-Larue MH, van Dongen JJM, Molina TJ, Cabeçadas J

Reference: Leukemia. 2007;21:207-14.

Link: https://pubmed.ncbi.nlm.nih.gov/17170731/

Clonality assessment
EuroClonality/BIOMED-2 primers

Improved reliability of lymphoma diagnostics via PCR-based clonality testing: report of the BIOMED-2 Concerted Action BHM4-CT98-3936

van Krieken JH, Langerak AW, Macintyre E, Kneba M, Hodges E, Sanz RG, Morgan GJ, Parreira A, Molina TJ, Cabeçadas J, Gaulard P, Jasani B, Garcia JF, Ott M, Hannsmann ML, Berger F, Hummel M, Davi F, Brüggemann M, Lavender FL, Schuuring E, Evans PA, White H, Salles G, Groenen PJ, Gameiro P, Pott C, Dongen JJM

Reference: Leukemia. 2007;21:201-6.

Link: https://pubmed.ncbi.nlm.nih.gov/17170732/

Clonality assessment
EuroClonality/BIOMED-2 primers

Human T-cell lines with well-defined T-cell receptor gene rearrangements as controls for the BIOMED-2 multiplex polymerase chain reaction tubes

Sandberg Y, Verhaaf B, van Gastel-Mol EJ, Wolvers-Tettero IL, de Vos J, Macleod RA, Noordzij JG, Dik WA, van Dongen JJM, Langerak AW.

Reference: Leukemia . 2007 Feb;21:230-7.

Link: https://pubmed.ncbi.nlm.nih.gov/17170727/

Wotherspoon criteria combined with B-cell clonality analysis by advanced polymerase chain reaction technology discriminates covert gastric marginal zone lymphoma from chronic gastritis.

Hummel M, Oeschger S, Barth TFE, Loddenkemper C, Cogliatti SB, Marx A, Wacker H-H, Feller AC, Bernd H-W, Hansmann M-L, Stein H, Möller P.

Reference: Gut/ 2006;55:782-787.

Link: https://pubmed.ncbi.nlm.nih.gov/16423889/

BIOMED-2 multiplex immunoglobulin/T-cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics

Sandberg Y, van Gastel-Mol EJ, Verhaaf B, Lam KH, van Dongen JJM, Langerak AW.

Reference: J Mol Diagn. 2005 Oct;7:495-503.

Link: https://pubmed.ncbi.nlm.nih.gov/16237219/

Validation of BIOMED-2 multiplex PCR tubes for detection of TCRB gene rearrangements in T-cell malignancies

Droese J, Langerak AW, Groenen PJ, Brüggemann M, Neumann P, Wolvers-Tettero IL, van Altena MC, Kneba M, van Dongen JJM.

Reference: Leukemia . 2004 Sep;18:1531-8.

Link: https://pubmed.ncbi.nlm.nih.gov/15284865/

TCRB gene rearrangements in childhood and adult precursor-B-ALL: frequency, applicability as MRD-PCR target, and stability between diagnosis and relapse

Van der Velden VHJ, Brüggemann M, Hoogeveen PG, De Bie M, Hart PG, Raff T, Pfeifer H, Luschen S, Szczepanski T, Van Wering ER, Kneba M, Van Dongen JJM.

Reference: Leukemia. 2004;18:1971-1980.

Link: https://pubmed.ncbi.nlm.nih.gov/15470492/

Rearranged T-cell receptor beta genes represent powerful targets for quantification of minimal residual disease in childhood and adult T-cell acute lymphoblastic leukemia

Brüggemann M, Van der Velden VHJ, Raff T, Droese J, Ritgen M, Pott C, Wijkhuijs AJ, Gokbuget N, Hoelzer D, Van Wering ER, Van Dongen JJM, Kneba M.

Reference: Leukemia. 2004;18:709-719.

Link: https://pubmed.ncbi.nlm.nih.gov/14961040/

Clonality analysis for antigen receptor genes: preliminary results from the Biomed-2 concerted action PL 96-3936

van Krieken JH, Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan GM, Kneba M, Macintyre E, van Dongen JJM

Reference: Hum Pathol. 2003;34:359-61.

Link: https://pubmed.ncbi.nlm.nih.gov/12733116/

EuroClonality/BIOMED-2 primers
Clonality assessment

Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936

van Dongen JJM, Langerak AW, Brüggemann M, Evans PA, Hummel M, Lavender FL, Delabesse E, Davi F, Schuuring E, García-Sanz R, van Krieken JH, Droese J, González D, Bastard C, White HE, Spaargaren M, González M, Parreira A, Smith JL, Morgan GJ, Kneba M, Macintyre E

Reference: Leukemia. 2003;17:2257-317.

Link: https://pubmed.ncbi.nlm.nih.gov/14671650/

EuroClonality/BIOMED-2 primers
Clonality assessment

Molecular immunoglobulin/T-cell receptor clonality analysis in cutaneous lymphoproliferations. Experience with the BIOMED-2 standardized polymerase chain reaction protocol

Sandberg Y, Heule F, Lam K, Lugtenburg PJ, Wolvers-Tettero ILM, Van Dongen JJM, Langerak AW.

Reference: Journal of Hematopathology . 2003 Jun;88:659-670.

Link: https://pubmed.ncbi.nlm.nih.gov/12801842/

Polymerase chain reaction-based clonality diagnosis of lymphoproliferative disorders

Van Dongen JJM, Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan G, Kneba M, Van Krieken JHJM, Macintyre EA.

Reference: The Hematology Journal 2002;3S2:31-33

Clonality analysis in malignant lymphoma: the BIOMED-2 experience

Langerak AW, San Miguel JF, Parreira A, Smith JL, Morgan G, Kneba M, Macintyre E, Van Krieken JHJM, Van Dongen JJM.

Reference: Histopathol 2002;41S2:506-508.

Link: https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2559.41.s2.65.x